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Sample preparation: Samples were prepared homogenizing 30 g in 275 mL of Buffered Peptone Water (BPW, Applichem, Darmsdadt, Germany) using a Stomacher 400, Seward, London, UK for 120 s at medium speed and serially diluted in the same diluent when needed.
To start the analysis, vials were rehydrated with 10 mL of sterile distilled water and paraffin oil and shaken until all the reagent was dissolved. Vials were inoculated with 1 mL of the sample homogenate and its serial dilutions, in parallel with the reference pour plate method. All analyses were performed in triplicate. Vials were incubated at 30 C for 30 h.
Sample preparation: Samples were prepared homogenizing 30 g in 275 mL of Buffered Peptone Water (BPW) using a Stomacher 400, Seward, London, UK for 120 s at medium speed and serially diluted in the same diluent. 153554b96e
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