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The expression of miR-675-5p was analyzed by real-time quantitative PCR (qRT-PCR). The effect of miR-675-5p on proliferation was evaluated through MTT and colony formation assays, and cell migration and invasion were evaluated through transwell assays. The expression of target proteins and downstream molecules was analyzed by western blotting and immunohistochemical staining. The luciferase reporter assay was used to assess the target genes of miR-675-5p in non-small cell lung cancer cells.
Workers have more bargaining power when they have more alternatives, form unions, or exercise democratic controls themselves as they do with minimum wage laws, or (on a smaller scale) when they run workers cooperatives. Alternatively (or in addition to), a basic income can be implemented that ensures that not working is a viable alternative. This prevents the conditions for my definition of wage slavery to be satisfied in the first place. 153554b96e
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